  1.1.1.B18 | construction of Rhodococcus expression vectors, derived from the Rhodococcus/Escherichia coli shuttle vector pRET1102, to express aadh. The plasmid pRET1102 can be transformed into many actinomycete strains, including Rhodococcus erythropolis. The transformation efficiency for a species closely related to Rhodococcus erythropolis is higher than that for other actinomycete strains. Promoters of various strengths, hsp, 1200rep, and TRR, are obtained from Gram-positive bacteria. The activity of TRR is stronger than that of hsp and 1200rep. The aadh-expressing plasmid pRET1172 with TRR can be transformed into many actinomycete strains to increase their AADH production. The Rhodococcus expression vector, pRET11100, constructed by removing aadh from the pRET1172 plasmid may be useful for bioconversion |
