EC Number   |
|---|
   1.11.1.14 | - |
| 1.11.1.14 | design of a synthetic gene encoding the N246A variant of DypB from Rhodococcus jostii strain RHA1 (Rh_DypB) is designed by back translation of the protein sequence, recombinant overexpression of His-tagged N246A mutant enzyme in Escherichia coli strain BL21(DE3), the enzyme is fully produced as folded holoenzyme, thus without the need for a further reconstitution step |
| 1.11.1.14 | enzyme cloning from strain NK-1, phylogenetic analysis |
| 1.11.1.14 | expressed in Escherichia coli BL21(DE3)pLysS cells |
| 1.11.1.14 | expressed in Escherichia coli BL21(DE3)pLysS cells, recombinant LiP protein accumulates in inclusion bodies as an inactive form |
| 1.11.1.14 | expression in Escherichia coli |
| 1.11.1.14 | expression in Saccharomyces cerevisiae |
| 1.11.1.14 | gene LPOA, recombinant expression of the codon-optimized gene in Escherichia coli strain W3110. The cloned expression vector pFLAG1 and the resulting plasmid pFLAG1-Y00262 are directly used for expression, Escherichia coli strain DH5x02alpha is used for plasmid propagation. The apoenzyme accumulates in inclusion bodies |
| 1.11.1.14 | homologous overexpression in Phanerochaete sordida YK-624 uracil auxotrophic mutant UV-64 |
| 1.11.1.14 | overexpression of isozyme H8 and W171 mutant in Escherichia coli |
