EC Number   |
|---|
   1.14.11.53 | - |
| 1.14.11.53 | a plasmid with the vector backbone pNIC28-Bsa4 encoding a hexahistidine-tagged ALKBH566292 construct is transformed into Escherichia coli BL21 (DE3) cells |
| 1.14.11.53 | expressed in Escherichia coli BL21(DE3) cells |
| 1.14.11.53 | expressed in Escherichia coli BL21(DE3) Rosetta cells |
| 1.14.11.53 | expression in Escherichia coli BL21 Star (DE3) |
| 1.14.11.53 | expression in the BL21 (DE3) Escherichia coli strain |
| 1.14.11.53 | mutants of human Alkbh5 are amplified by PCR and subcloned into a modified pET-28a (Novagen) vector encoding a tobacco etch virus protease recognition site. The final clones are verified by DNA sequencing. All of the recombinant plasmids are transformed into Escherichia coli strain BL21(DE3) |
| 1.14.11.53 | the human ALKBH5 catalytic AlkB domain (residues 74294) is subcloned into pET28a-MHL vector and expressed. The cloned vector is transformed into Escherichia coli BL21-V2R-pRARE2. Recombinant protein is produced at 16°C as an N-terminal His-tagged protein |
| 1.14.11.53 | Wild-type and various mutant enzymes cloned into pET-15b are expressed in Escherichia coli strain BL21 |
