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EC Number Cloned (Commentary)
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8expressed in COS-1 cells
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8expressed in Escherichia coli BL21-AI cells
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8gene TMLH, recombinant overexpression of active and stable TMLH as a His-tagged maltose-binding protein fusion in Escherichia coli strain BL21-AI. Coexpression of the enzyme with chaperones GroES/EL increases the expression level by one order of magnitude
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8recombinant expression of the enzyme fused to MBP
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
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