EC Number   |
|---|
   1.3.7.15 | expressed in Escherichia coli BL21(DE3) cells |
| 1.3.7.15 | genes bchX and bchY, recombinant expression of GST-tagged BchX and BchY in Escherichia coli |
| 1.3.7.15 | genes bchX, bchY, and bchZ, detailed phylogenetic analysis and tree of BchXYZ |
| 1.3.7.15 | recombinant overexpression of enzymatically active subunit (BchY/BchZ)2 and BchX2 subcomplexes in Escherichia coli strain BL21(DE3), expression of N-terminally His6-tagged BchY, N-terminally thioredoxin/His/S-tagged BchX2, and untagged BchZ under the control of individual promoters. Supplementation with Fe(III)citrate and L-cysteine as sources of iron and sulfur, respectively, as media constituents has significant influence on the in vivo assembly of [4Fe-4S] clusters, growth temperature is 17°C |
| 1.3.7.15 | reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment |
