EC Number |
---|
1.5.1.2 | - |
1.5.1.2 | a proline oxidase antisense vector is constructed by amplifying a part of the proline oxidase cDNA and cloning it in the mammalian expression vector pCI |
1.5.1.2 | cloned into a pMCSG7 vector, generating an expression clone of a fusion protein with an N-terminal His6-tag and a TEV protease recognition site for expression in Escherichia coli |
1.5.1.2 | cloned into the pET28a+ vector with a His6 tag at the N-terminus for expression in the Escherichia coli strain B834DE3 |
1.5.1.2 | expressed in Escherichia coli BL21(DE3) cells |
1.5.1.2 | expressed in Escherichia coli BL21(DE3) pLysS cells |
1.5.1.2 | expression in Escherichia coli |
1.5.1.2 | expression in Esherichia coli |
1.5.1.2 | fusion of enzyme promotor to beta-glucuronidase |
1.5.1.2 | gene PYR2, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) pLysS |