EC Number   |
|---|
   1.6.2.4 | - |
| 1.6.2.4 | canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid |
| 1.6.2.4 | chimeric protein with His-tag |
| 1.6.2.4 | coexpressed together with a form of cytochrome P450 in Salmonella typhimurium TA1538 |
| 1.6.2.4 | coexpression with CYP3A4 in Spodoptera frugiperda and Trichoplusia ni cell lines |
| 1.6.2.4 | coexpression with two differently modified CYP2D6 cDNAs in Escherichia coli, 17alpha-2D6 + pJR7 and ompA-2D6 + pJR7 |
| 1.6.2.4 | DNA and amino acid sequence determination and analysis, sequence comparisons, the enzyme shows highly conserved exon/intron organization structure, functional expression in Escherichia coli membranes |
| 1.6.2.4 | expressed in Bacillus subtilis DB104 under the transcriptional control of an IPTG-inducible fusion promoter of PgroE and Ptac. The expressed rCPR is released into the culture medium after sporulation by autolysis of the host cell. It is associated with and displayed on the spore surfaces |
| 1.6.2.4 | expressed in Escherichia coli BL21 cells |
| 1.6.2.4 | expressed in Escherichia coli BL21 Star cells |
