EC Number |
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1.8.99.2 | - |
1.8.99.2 | expressed in Eschericha coli as the N-terminal reductase domain (AcAPR1-N) and the C-terminal glutaredoxin domain (AcAPR1-C). Recombinant full-length ATP sulfurylase (AcATPS1) and recombinant full length adenosine-5'-phosphosulfate reductase (AcAPR1) from Allium cepa can form a physical association |
1.8.99.2 | expressed in Escherichia coli |
1.8.99.2 | expressed in Escherichia coli JM109 cells |
1.8.99.2 | expressed in Zea mays |
1.8.99.2 | expression in Escherichia coli |
1.8.99.2 | expression in Escherichia coli of a recombinant His-tagged protein and several mutants |
1.8.99.2 | expression in Escherichia coli of a tagged recombinant protein |
1.8.99.2 | full-length AprBA sequences from 20 phylogenetically distinct sulfate-reducing prokaryotes and sulfuroxidizing bacteria species are used for homology modeling. Based on the comparative models, there are no significant structural differences between dissimilatory APS reductases from sulfate-reducing prokaryotes and sulfur-oxidizing bacteria. This might be indicative for a similar catalytic process of APS reduction/sulfite oxidation |
1.8.99.2 | gene apsA, PCR-based DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli |