EC Number |
---|
3.1.1.53 | expressed as GFP-fused secretion protein in Sf9 cells |
3.1.1.53 | expressed as HaloTag fusion protein in Escherichia coli O157:H7 Stx cells |
3.1.1.53 | expressed in COS-7 cells |
3.1.1.53 | expressed in COS-7 cells, both the larger and smaller Lse subunit |
3.1.1.53 | expressed in Escherichia coli BL21(DE3) Origami B cells |
3.1.1.53 | expression in COS7 cell |
3.1.1.53 | expression in E. coli BL21 |
3.1.1.53 | expression in Escherichia coli |
3.1.1.53 | expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3). SsneuA can restore both CMP-Neu5Ac synthetase and O-acetylesterase activities in complemented enzyme-deficient Escherichia coli strains, but the activities in the complemented strain are different from those in the wild-type Escherichia coli strain. However, the Streptococcus suis O-acetylesterase domain alone is unable to act on intracellular O-acetyl-Neu5Ac in Escherichia coli |
3.1.1.53 | genotyping, exome sequencing, in HEK293 T cells overexpressed recombinant enzyme can be secreted by the transfected lymphocates, in the contrary to the physiological state of the enzyme |