Search Temperature Stability [°C]

1.1.1.346

0

38

temperature stability of isoform 2,5-diketo-D-gluconate reductase I is measured by storing the enzyme in 0.1 M Tris-HC1 buffer (pH 7.0) for 1 h. The activity of the enzyme decreases gradually with temperature from 0�C to 27�C, falls rapidly above 27�C, and is lost completely at about 38�C. The remaining activity at 27�C is about 77% of that at 0�C

1.1.1.346

40

80

after approximately 10 min of deactivation, enzyme activity attains a constant value, independently of deactivation temperature (40-80�C). This value corresponds to the 30% of native activity