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EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
temperature stability of isoform 2,5-diketo-D-gluconate reductase I is measured by storing the enzyme in 0.1 M Tris-HC1 buffer (pH 7.0) for 1 h. The activity of the enzyme decreases gradually with temperature from 0C to 27C, falls rapidly above 27C, and is lost completely at about 38C. The remaining activity at 27C is about 77% of that at 0C
after approximately 10 min of deactivation, enzyme activity attains a constant value, independently of deactivation temperature (40-80C). This value corresponds to the 30% of native activity
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