EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   1.14.14.3 | -999 |
- |
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature |
671414 |
| 1.14.14.3 | -999 |
- |
the recombinant enzyme shows reduced thermal stability in absence of chaperone Hsp100 after expression in an Escherichia coli clpA-mutant strain, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo |
658147 |
| 1.14.14.3 | -999 |
- |
the recombinant enzyme shows unaffected thermal stability after expression in an Escherichia coli clpA-mutant strain lacking chaperone Hsp100, carbonyl cyanide 3-chlorophenylhydrazone highly decreases the thermal stability of the enzyme in vivo to in vitro level, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo |
658147 |
| 1.14.14.3 | 30 |
37 |
half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C |
728017 |
| 1.14.14.3 | 35 |
- |
stable below |
348582 |
| 1.14.14.3 | 37 |
- |
comparison of stability of wild-type enzyme and gene-fusion monomeric enzyme |
348549 |
| 1.14.14.3 | 37 |
- |
wild-type enzyme has a 90% reduction in activity after 92 min. Comparable loss for the Y151W mutant after only 11 min |
702318 |
| 1.14.14.3 | 40 |
- |
wild-type enzyme and mutanbt A75G: loss of 60% activity within 50 min, mutants C106V and C106V/A75G show increased thermolability loosing 99% and 90% activity, respectively |
657939 |
| 1.14.14.3 | 43.5 |
- |
inactivation in vitro, temperature-stable enzyme |
658147 |
| 1.14.14.3 | 43.5 |
- |
inactivation, temperature-labile enzyme |
658147 |
