EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.7.1.36 | 20 |
30 |
the enzyme is stable at 20-25°C for 80 min and at optimum temperature 30°C for 40 min |
738399 |
2.7.1.36 | 37 |
- |
approx. 50% and 70% loss of activity after 40 min and 90 min, respectively, H20P/V377I double mutant is only slightly more temperature sensitive |
641456 |
2.7.1.36 | 44 |
- |
10 min, residual activity of wild-type 50%, of mutant A141C 85% |
691055 |
2.7.1.36 | 47 |
- |
10 min, residual activity of wild-type 15%, of mutant A141C 55% |
691055 |
2.7.1.36 | 50 |
- |
1 h, little inactivation |
641443 |
2.7.1.36 | 50 |
- |
approx. 50% and 90% loss of activity after 10 min and 30 min, respectively, H20P/V377I double mutant is only slightly more temperature sensitive |
641456 |
2.7.1.36 | 50 |
- |
rate constant for inactivation of wild-type and V377I mevalonate kinase: 0.026 and 0.031/min |
641454 |
2.7.1.36 | 70 |
- |
no loss of activity after 24 h |
641450 |
2.7.1.36 | 80 |
- |
1 h, 60% loss of activity |
641443 |
2.7.1.36 | 83 |
- |
10 min, residual activity of wild-type 50%, mutants C107S and C281S 20%, mutant C107S/C281S 15%, mutants C107A and C281A 20%, mutant C107A/C281A 18% |
691055 |