EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.1.99.31 | 0.11 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme G81D |
667577 |
| 1.1.99.31 | 0.15 |
- |
3-Phenyllactate |
pH 7.5, 20°C, mutant enzyme G81A |
667577 |
| 1.1.99.31 | 0.19 |
- |
2-hydroxyhexanoate |
pH 7.5, 20°C, mutant enzyme G81A |
667577 |
| 1.1.99.31 | 0.22 |
- |
O2 |
pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH |
667577 |
| 1.1.99.31 | 0.23 |
- |
(S)-Mandelate |
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 |
678128 |
| 1.1.99.31 | 0.23 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme R277G |
667572 |
| 1.1.99.31 | 0.26 |
- |
2-Hydroxyoctanoate |
pH 7.5, 20°C, mutant enzyme G81A |
667577 |
| 1.1.99.31 | 0.27 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme R277L |
667567 |
| 1.1.99.31 | 0.27 |
- |
2-hydroxyvalerate |
pH 7.5, 20°C, mutant enzyme G81A |
667577 |
| 1.1.99.31 | 0.27 |
- |
3-indolelactate |
pH 7.5, 20°C, mutant enzyme G81A |
667577 |
