2.2
-
NADPH
mutant enzyme F22Y/S233T/R235S/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C
2.6
-
ethyl 2-methylacetoacetate
at pH 7.0 and 30°C
3.3
-
NADPH
mutant enzyme F22Y/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C
7
-
NADPH
mutant enzyme F22Y/K232G/R235G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C
7.3
-
NADPH
mutant enzyme K232G/R238H, in 100 mM Bis-Tris, pH 7.0, at 25°C
8.3
-
2,5-didehydro-D-gluconate
at pH 6.4 and 25°C
18.3
-
NADPH
mutant enzyme F22Y/K232G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C
18.3
-
NADPH
wild type enzyme, in 100 mM Bis-Tris, pH 7.0, at 25°C