EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference  |
|---|
    4.2.99.20 | 0.18 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
25°C, mutant enzyme D210A |
685225 |
| 4.2.99.20 | 0.29 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
25°C, mutant enzyme S86A |
685225 |
| 4.2.99.20 | 5.8 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
25°C, mutant enzyme H232A |
685225 |
| 4.2.99.20 | 147 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
25°C, wild-type enzyme |
685225 |
| 4.2.99.20 | 167 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
pH 7.0, 37°C |
685225 |
| 4.2.99.20 | 0.00058 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
H232A mutant protein |
702326 |
| 4.2.99.20 | 0.021 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
H232K mutant protein |
702326 |
| 4.2.99.20 | 0.047 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
R90A mutant protein |
702326 |
| 4.2.99.20 | 0.056 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
W147A mutant protein |
702326 |
| 4.2.99.20 | 0.18 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
D210A mutant protein |
702326 |
