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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
1.1.1.274
1.23
-
NADH
F22Y/A272G isoenzyme A mutant
440311
1.1.1.274
2.17
-
NADPH
F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant
440311
1.1.1.274
2.6
-
ethyl 2-methylacetoacetate
pH 7.0, 30°C
440312
1.1.1.274
2.6
-
ethyl-2-methylacetoacetate
-
440312
1.1.1.274
2.83
-
NADH
isoenzyme A, wild-type
440311
1.1.1.274
3.33
-
NADPH
K232G/R238H isoenzyme A mutant
440311
1.1.1.274
7
-
NADPH
F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant
440311
1.1.1.274
7.33
-
NADPH
F22Y/K232G/R238H/A272G isoenzyme A mutant
440311
1.1.1.274
8.33
-
2,5-didehydro-D-gluconate
-
440304
1.1.1.274
8.5
-
NADH
F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant
440311
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