EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.1.1.85 | -999 |
- |
more |
the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126 |
657223 |
| 1.1.1.85 | 0.0025 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme D241A, at pH 7.6 and 20°C |
737729 |
| 1.1.1.85 | 0.0025 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme K185A, at pH 7.6 and 20°C |
737729 |
| 1.1.1.85 | 0.003 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme D292N, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.016 |
- |
3-(2'-methylthio)ethylmalate |
wild-type, pH not specified in the publication, temperature not specified in the publication |
725437 |
| 1.1.1.85 | 0.0167 |
- |
3-(2'-methylthio)ethylmalate |
wild-type, pH not specified in the publication, temperature not specified in the publication |
725437 |
| 1.1.1.85 | 0.017 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme Y181A, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.023 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme R146K, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.03 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme R146A, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.033 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme E270A, at pH 7.6 and 20°C |
737729 |
