EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.99.31 | 0.03 |
- |
(R,S)-p-chloromandelate |
mutant enzyme H274G, in presence of 20 mM imidazole |
667566 |
1.1.99.31 | 0.03 |
- |
2-Hydroxyisovalerate |
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach |
667565 |
1.1.99.31 | 0.03 |
- |
O2 |
pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach |
667577 |
1.1.99.31 | 0.04 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme R165G/R277K |
667572 |
1.1.99.31 | 0.05 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme G81V |
667577 |
1.1.99.31 | 0.05 |
- |
O2 |
pH 7.5, 20°C, mutant enzyme G81D |
667577 |
1.1.99.31 | 0.07 |
- |
O2 |
pH 7.5, 20°C, mutant enzyme G81V |
667577 |
1.1.99.31 | 0.09 |
- |
(S)-Mandelate |
mutant enzyme H274G, in presence of 20 mM imidazole |
667566 |
1.1.99.31 | 0.1 |
- |
2-Hydroxybutyrate |
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach |
667565 |
1.1.99.31 | 0.1 |
- |
2-Hydroxybutyrate |
pH 7.5, 20°C, wild-type enzyme |
667577 |