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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
4.6.1.24
-999
-
more
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W
664601
4.6.1.24
-999
-
more
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV)
663882
4.6.1.24
0.7
-
poly(I)
25°C, pH 6.2, mutant enzyme E54Q
663837
4.6.1.24
38
-
GpU
-
24192
4.6.1.24
62
-
GpG
-
24192
4.6.1.24
72
-
5'-OHGGGACAGUAUUUG-3'
wild-type
683904
4.6.1.24
96
-
GpA
-
24192
4.6.1.24
126
-
5'-pGGGACAGUAUUUG-3'
R169K mutant
683904
4.6.1.24
180
-
5'-pGGGACAGUAUUUG-3'
wild-type
683904
4.6.1.24
189
-
poly(I)
25°C, pH 6.2, wild-type enzyme
663837
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