EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.4.99.25 | -999 |
- |
more |
turnover numbers are determined for wild-type and mutant forms of yeast tRNAPhe. The 7 base T-loop can be expanded or contracted by 1 base and still retains activity, albeit with a 30fold reduction in kcat |
702196 |
5.4.99.25 | 0.011 |
- |
tRNA uridine55 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R |
702203 |
5.4.99.25 | 0.012 |
- |
tRNA uridine55 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M |
702203 |
5.4.99.25 | 0.11 |
- |
tRNA uridine55 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme |
702203 |
5.4.99.25 | 0.11 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A |
648867 |
5.4.99.25 | 0.12 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme |
648867 |
5.4.99.25 | 0.16 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A |
648867 |
5.4.99.25 | 0.18 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V |
648867 |
5.4.99.25 | 0.18 |
- |
tRNA uridine55 |
wild-type enzyme |
661179 |
5.4.99.25 | 0.23 |
- |
tRNA uridine55 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G |
702203 |