EC Number   |
Cofactor |
Reference |
|---|
    1.1.1.245 | NAD+ |
- |
12556, 246371, 286378, 667621 |
| 1.1.1.245 | NAD+ |
isoenzyme II |
286383 |
| 1.1.1.245 | NAD+ |
temperature-induced conformational change is associated with the flexible loops directly involved in the substrate and coenzyme binding |
667621 |
| 1.1.1.245 | NADH |
enzyme exhibits linearity of NAD(H) binding at pH 9.8 and at moderate ionic strength, in addition to positive cooperativity at pH 7.8 and 6.8, and at pH 9.8 in the presence of salt. NADH binding is positively cooperative below 20°C and negatively cooperative at 4050°. Steady-state kinetic measurements show that SsADH displays standard MichaelisMenten kinetics between 35°C and 45°C, but exhibits positive and negative cooperativity for NADH oxidation below and above this range of temperatures, respectively. Mutant N249Y displays non-cooperative behavious in coenzyme binding under the same experimental conditions used for the wild-type enzyme |
667502 |
| 1.1.1.245 | NADP+ |
less than 5% activity than with NAD+ |
12556 |
| 1.1.1.245 | NADP+ |
weaker activity than with NAD+ |
286378 |
