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purified wild-type and mutant enzymes in complex with NADPH, hanging drop vapour diffusion method, mixing of protein solution containing 2 mM NADPH with reservoir solution containing 0.1 M Tris-HCl, pH 7.0, 0.2 M NH4Ac, and 30% v/v pentaerythritol ethoxylate, 5 days, 20°C, platinum-derivatives are obtained by soaking native crystals for 12 h in mother liquor plus 10 mg/ml K2Pt(NO2)4, X-ray diffraction structure determination and analysis at 2.74-4.3 A resolution, selenium-based single-wavelength anomalous dispersion, modeling
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