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EC Number
Crystallization
Reference
(dI.Q132N)2dIII trimer
; dI2dIII complex with bound NAD+ and NADP+, NADH and NADPH, and ADP-ribose and NADPH
crystal structure of domain I i.e. alpha1 subunit, with and without bound NADH, 1.8 A resolution without NADH, 1.9 A with NADH bound
crystal structure of domainI/domain III complex
dI2dIII1 complex with bound NAD+ and 1,4,5,6-tetrahydronicotinamide adenine dinucleotide phosphate the dI2dIII1 complex with bound ,4,5,6-tetrahydronicotinamide adenine dinucleotide and NADP+. dI is the NAD(H)-binding component of transhydrogenase. dIII is the NADP(H)-binding component
dIII domain in the presence of NADPH
dimers of dI domains using hanging-drop vapor diffusion method
domain dIII, domain dI dimer and dI2dIII complex in the presence of limiting NADH using the vapor diffusion method
hanging-drop method, crystal structures of the NAD(H)-binding domain I of transhydrogenase in the absence as well as in the presence of oxidized and reduced substrate. The structures are determined at 1.9-2.0 A resolution; NAD(H)-binding domain I in the absence and in the presence of NAD+ or NADH, hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M tri-sodium citrate dihydrate (pH 5.6) and 30% (w/v) PEG 4000
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