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Results 1 - 10 of 10
EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30crystallization of the enzyme in the apo form and in the holo form with acetate as a substrate analogue, method screening, mother liquor consists of 30% w/v PEG 4000, 0.2 M sodium acetate trihydrate and 100 mM Tris-HCl, pH 8.5, at 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular-replacement method
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30crystals of ternary complex of HBDH-NAD+-L-3-hydroxybutyrate and the binary complex of HBDH-NAD+. The former structure shows a closed-form conformation, which is considered an active form for catalysis, while the latter stays mostly in a open-form conformation. Crystals of mutants T190S and T190A
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30hanging drop vapour diffusion method with 17-20% polyethylene glycol 1500, 0.1 M Tris-HCl, pH 7.1, 0.2 mM CaCl2 and 10 mM acetoacetate
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30hanging-drop vapour-diffusion method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 A. The crystals are most likely to contain two tetrameric subunits in the asymmetric unit
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30in complex with NAD+, malonate, or methylmalonate, hanging drop vapor diffusion method, using 30-34% (w/v) PEG 4000
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30in the presence of the substrate D-3-hydroxybutyrate and the cofactor NAD+ at the optimum pH for the catalytic reaction. At 277 and 293 K using the hanging-drop vapour-diffusion method, to 2.3 A resolution. Structure is isomorphous to that of the complex with the substrate analogue acetate
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30recombinant enzyme, hanging drop vapor diffusion method, 0.003 ml of HBDH, 10 mg/ml, is mixed with an equal volume of crystallization buffer containing 17-20% PEG 1500, 0.1 M Tris-HCl, pH 7.1, 0.2 mM CaCl2, and 10 mM acetoacetate, room temperature/22°C, three different crystal forms, X-ray diffraction structure determination and analysis at resolutions between 1.9 and 2.1 A
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30sitting drop vapor diffusion method at 20°C, structure determined at a resolution of 1.8 A in complex with NAD(H)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.30structures of enzyme complexed to NAD+:acetoacetate and NAD+:3-oxovalerate
Results 1 - 10 of 10