1.1.1.406 | purified recombinant enzyme, free or with bound 1,2-pentanediol or meso-erythritol, hanging drop vapour diffusion method, 0.001 ml of 0.45 mM protein with 1 mM NAD+ or NADH, in 20 mM Bis-Tris, pH 6.5, is mixed with 0.001 ml of reservoir solution containing 100 mM MES, pH 5.5-5.9, 200 mM MgCl2, and 10-20% w/v methoxy poly(ethylene glycol), equilibration against 1 ml reservoir solution, 1 week, X-ray diffraction structure determnination and analysis at 1.25-195 A resolution |