EC Number   |
|---|
   1.14.12.18 | - |
| 1.14.12.18 | crystal structure of the BPDOB356/2,6-dichlorobiphenyl complex is determined at 2.4 A resolution |
| 1.14.12.18 | crystal structure of the BPDOB3562,6-dichlorobiphenyl complex is determined at 2.4 A resolution |
| 1.14.12.18 | hanging drop vapour diffusion method using 20% (w/v) polyethylene glycol 3350, 1 M NaCl and 0.1 M ZnCl2 at 6°C |
| 1.14.12.18 | purified recombinant BPDOLB400, sitting drop vapour diffusion method, anaerobic conditions, method optimization, 0.002 ml of 8 mg/ml protein in 25 mM HEPES, pH 7.3, containing 0.25 mM ferrous ammonium sulfate, and 2 mM dithiothreitol, is mixed with 0.002 ml of reservoir solution containing 20-25% w/v PEG 8000 or PEG 5000 MME, 50 mM PIPES, pH 6.5, 100 mM ammonium acetate, 5% v/v glycerol, and 0.2% w/v agarose, successful crystallization only occurs at pH 6.5, X-ray diffraction structure determination and analysis at 2.4-2.8 A resolution |
| 1.14.12.18 | sitting drop vapor diffusion method, using 20-25% (w/v) PEG 8000 or PEG 5000 MME, 50 mM PIPES pH 6.5, 100 mM ammonium acetate, 5% (v/v) glycerol and 0.2% (w/v) agarose |
| 1.14.12.18 | sitting drop vapor diffusion method, using 2025% (w/v) PEG 8000 or PEG 5000 MME, 50 mM PIPES (pH 6.5), 100 mM ammonium acetate, 5% (v/v) glycerol, and 0.2% (w/v) agarose |
| 1.14.12.18 | terminal oxygenase component of the biphenyl dioxygenase, BphA1A2 in substrate-free and biphenyl complex forms, cocrystallization method |
