  1.14.13.101 | purified recombinant isozyme PA N-oxygenase (ZvFMOa), mixing of 6.5 mg/ml protein in 20 mM glycine-NaOH, pH 9.0, and 1 mM DTT with an equal amount of precipitant solution containing 20 mM bis-tris propane-NaOH, pH 7.5, 200 mM NaNO3, 22% w/v PEG 3000, 1 mM TMA-HCl, and 1 mM NADP+, equilibration against reservoir solution, 18°C, X-ray diffraction structure determiantion and analysis at 3.0 A resolution |
| 1.14.13.101 | purified recombinant isozyme PA N-oxygenase (ZvPNO) with bound FAD or in complex with FAD and NADP+, hanging drop vapour diffusion method, mixing of 5.6 mg/ml protein in 20 mM glycine-NaOH, pH 9.0, and 1 mM DTT with an equal amount of precipitant solution containing 20 mM Tris-HCl, pH 7.0, 200 mM MgCl2, and 15% w/v PEG 3350, with or without 1 mM NADP+, equilibration against reservoir solution, 18°C, X-ray diffraction structure determiantion and analysis at 1.6-1.89 A resolution |
