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EC Number	Crystallization (Commentary)
1.14.13.217	purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 7.5-15 mg/ml protein in 20 mM TrisHCl pH 8.0, 300 mM NaCl, 10% glycerol, with 0.001 ml of reservoir solution containing 3.5 M sodium formate pH 7.0, and equilibration against 0.05 ml of reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit

EC Number

Crystallization (Commentary)

1.14.13.217

purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 7.5-15 mg/ml protein in 20 mM TrisHCl pH 8.0, 300 mM NaCl, 10% glycerol, with 0.001 ml of reservoir solution containing 3.5 M sodium formate pH 7.0, and equilibration against 0.05 ml of reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit

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Release 2023.1 (January 2023)