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EC Number	Crystallization (Commentary)
1.5.99.15	hanging drop vapor diffusion method, using 0.8-1.2 M ammonium sulfate as precipitant
1.5.99.15	purified recombinant N-terminally His6 -tagged enzyme by hanging drop vapor diffusion method, mixing of 0.003 ml of 13 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5% glycerol, and 4 mM DTT, with reservori solution containing FMN, method optimization, 7-14 days at room temperature, X-ray diffraction structure determination and analysis at 1.9 A resolution

Results 1 - 2 of 2

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Release 2023.1 (January 2023)