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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.8.4.14hanging drop vapor diffusion method. Crystals from native and mutant enzyme grew at 20°C within 2 days. To obtain a substrate-bound form, the crystals of the C84S mutant enzyme are soaked with the mother liquid containing 10 mM Met-R-O at 22°C for 1 h
Display the word mapDisplay the reaction diagram Show all sequences 1.8.4.14hanging-drop vapor diffusion method at 20°C. Crystal structure is determined in native form and in a complex with the substrate
Display the word mapDisplay the reaction diagram Show all sequences 1.8.4.14in complex with methionine sulfoxide, to 1.25 A resolution. Residue C118 is the catalytic Cys on which a sulfenic acid is formed
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