EC Number   |
|---|
    2.7.1.25 | - |
| 2.7.1.25 | APSK isoform 1 in complex with beta,gamma-imidoadenosine-5'-triphosphate, Mg2+, and adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 200 mM MgCl2, and 15-17.5% (w/v) PEG 2000 |
| 2.7.1.25 | crystal structures of the PAPSS1 APS-kinase domain in complex with APS and in complex with the products PAPS and ADP are solved, both structures of isolated domain, obtained in different crystal forms, reveal symmetrical dimers |
| 2.7.1.25 | crystals are grown at room temperature using hanging drops containing equal volumes of protein and reservoir solution |
| 2.7.1.25 | crystals are grown by hanging drop vapor diffusion at 25°C in 40% (v/v) ethanol, 1.0% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM MgATP |
| 2.7.1.25 | crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A |
| 2.7.1.25 | hanging drop vapor diffusion method |
| 2.7.1.25 | hanging-drop vapor diffusion method |
| 2.7.1.25 | in complex with adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.25, 200 mM MgCl2, and 1517.5% (w/v) PEG 2000 |
| 2.7.1.25 | in complex with AMP-PNP and adenosine-5'-phosphosulfate, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate, pH 6.5, 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) PEG 8000 |
