EC Number   |
Protein Variants |
Reference |
|---|
  1.13.11.48 | H102Q |
Km for the heteroaromatic substrate is increased only 2.8fold, and kcat is reduced 2.3fold |
-, 673207 |
| 1.13.11.48 | H102Q/C69S |
site-directed mutagenesis, mutant apparent kinetic parameters compared to the wild-type enzyme |
673207 |
| 1.13.11.48 | H251A |
2258fold lower specific activity than wild-type |
-, 658042 |
| 1.13.11.48 | S101A |
about 21fold increase in the Km for the organic substrate |
-, 673207 |
| 1.13.11.48 | S101A/C69S |
site-directed mutagenesis, mutant apparent kinetic parameters compared to the wild-type enzyme |
673207 |
| 1.13.11.48 | S220N |
does not drastically influence the kinetic parameters of the enzyme for the organic substrate, but it causes a 3.5fold decrease in Km for O2 and a 6.2fold decrease in kcat for O2 |
673207 |
| 1.13.11.48 | S220N/C69S |
site-directed mutagenesis, mutant apparent kinetic parameters compared to the wild-type enzyme |
673207 |
| 1.13.11.48 | Y196A |
increased Km value |
673207 |
| 1.13.11.48 | Y196A/C69S |
site-directed mutagenesis, mutant apparent kinetic parameters compared to the wild-type enzyme |
673207 |
| 1.13.11.48 | Y196K |
increased Km value |
673207 |
