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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 1.7.1.B2F70A/F108Y the mutations confer high levels of 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide activity and also substantially improve activity with 5-(aziridin-1-yl)-2,4-dinitrobenzamide and metronidazole. The mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2F70A/F108Y the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2N71S site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme 743709
Display the reaction diagram Show all sequences 1.7.1.B2T41L site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme 743709
Display the reaction diagram Show all sequences 1.7.1.B2T41L the mutation does not increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2T41L/F70A the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2T41L/F70A/F108Y the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2T41L/F70A/N71S the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2T41L/F70A/N71S/F108Y the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
Display the reaction diagram Show all sequences 1.7.1.B2T41L/N71S the mutations do not increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide 764166
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