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Results 1 - 10 of 31 > >>
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35more constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity 655929
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35more expression in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli 655363
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35D340F/Y418F increase in temperature stability 655127
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35D340F/Y418I increase in temperature stability 655127
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35T416V/T417V increase in temperature stability 655127
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35S231K increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively 639218
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35E277Q substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively 639216
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35E277A substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively 639216
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35D276E substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively 639216
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.35E277G substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively 639216
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