EC Number   |
Protein Variants |
Reference  |
|---|
   1.1.99.B3 | C130A |
FAD binding site, FAD covalently attached |
698829 |
| 1.1.99.B3 | H70A |
FAD binding site, FAD covalently attached |
698829 |
| 1.1.99.B3 | H70A/C130A |
no activity, lack of essential FAD cofactor |
698829 |
| 1.1.99.B3 | Y310A |
larger amount of carbohydrates |
698829 |
| 1.1.99.B3 | W351F |
the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates |
-, 724616 |
| 1.1.99.B3 | Y300A |
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates |
-, 724616 |
| 1.1.99.B3 | Y300N |
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates |
-, 724616 |
| 1.1.99.B3 | A38V |
the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme |
-, 740204 |
| 1.1.99.B3 | A38V/S388N |
the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme |
740204 |
| 1.1.99.B3 | E247A |
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme |
-, 740204 |
