EC Number |
Protein Variants |
Reference |
---|
1.14.11.51 | D233A |
reduced activity of the recombinant mutant protein |
736883 |
1.14.11.51 | D186A |
inactive |
742235 |
1.14.11.51 | D233A |
residue plays an important role in substrate recognition and catalysis |
764114 |
1.14.11.51 | H231A |
residue plays an important role in substrate recognition and catalysis |
764114 |
1.14.11.51 | H287A |
residue plays an important role in substrate recognition and catalysis |
764114 |
1.14.11.51 | R338A |
residue plays an important role in substrate recognition and catalysis |
764114 |
1.14.11.51 | R344A |
mutation in highly conserved residue, strong decrease in demethylation ability |
764114 |
1.14.11.51 | Y184A |
mutation in highly conserved residue, strong decrease in demethylation ability |
764114 |
1.14.11.51 | C118A |
m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity |
764135 |
1.14.11.51 | C118A/C129A |
about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity |
764135 |