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EC Number
Protein Variants
Commentary
Reference
Display the reaction diagram Show all sequences 1.14.13.155
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construction of an artificial self-sufficient P450-type monoterpene hydroxylase by fusing the wild-type P450SMO reductase domain and the P450cam(Y96F/V247L) domain to a linker region (G4S)4. The resultant chimeric P450 enzyme, chimeric P450cam (Y96F/V247L)-P450SMO red F1-F4, catalyzes the hydroxylation of (-)-limonene and (-)-alpha-pinene as well as camphor, which are all inactive for the wild-type enzyme P450SMO
745560
Display the reaction diagram Show all sequences 1.14.13.155
Y96F/V247L
mutant P450cam, shows altered substrate specificity compared to the wild-type enzyme
745560
Results 1 - 2 of 2
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