Search Protein Variants

1.5.3.10

D552A

mutation leads to increased formaldehyde release

1.5.3.10

H225Q

modest changes in kinetic parameters, no stabilization of FADH2-iminium charge-transfer complex

1.5.3.10

H225Q

site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme

1.5.3.10

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complete removal of 5,10-CH2-THF synthase domain through expression of a truncated version generates a mutant enzyme unable to avoid hydrolysis of the imine species generated following amine oxidation

1.5.3.10

Y259F

able to oxidize substrate, steady-state turnover is attenuated 200fold, rate of FAD reduction is substantially impaired, no stabilization of FADH2-iminium charge-transfer complex

1.5.3.10

Y259F

site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme