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the C-terminal extension of the enzyme is not required for the selection of G15 as the site of base exchange. Truncated forms of Pyrococcus furiosus TGT retain their specificity for guanine exchange at position 15. Deletion of the PUA domain causes a 4fold drop in the observed kcat and results in a 75fold increased Km for tRNAAsp compared with full-length TGT
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