EC Number   |
Protein Variants |
Reference |
|---|
   7.1.1.1 | A246C |
reverse activity stronger affected than cyclic activity |
658003 |
| 7.1.1.1 | A253C |
reverse activity stronger affected than cyclic activity |
658003 |
| 7.1.1.1 | A348C |
mutation introduced into a cysteine-free mutant enzyme, mutant shows markedly reduced activity |
392663 |
| 7.1.1.1 | A390C |
mutation introduced into a cysteine-free mutant enzyme |
392663 |
| 7.1.1.1 | A398C |
the mutant with wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme |
733772 |
| 7.1.1.1 | A432C |
mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 150% higher reaction rate than wild-type domain III/R. rubrum domain I mixture |
392667 |
| 7.1.1.1 | A432C |
the mutant shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme |
733772 |
| 7.1.1.1 | C292T/C339T/C395S/C397T/C435S |
cysteine of the alpha subunits replaced, similar activity as wild-type |
392662 |
| 7.1.1.1 | C292T/C339T/C395S/C397T/C435S/C147S/C260S |
all 7 cysteines of the enzyme, 5 localized in the alpha subunit and 2 in the beta subunit, are replaced, the cysteine-free mutant shows about 5fold more activity in the reduction of acetylpyridine adenine dinucleotide by NADH than wild-type, the cyclic reduction of acetylpyridine adenine dinucleotide by NADH via NADPH is 2-2.5fold more activ |
392662 |
| 7.1.1.1 | D135N |
mutation has no effect in binding affinity of either NAD+ or NADH |
674550 |
