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rapid immobilization with the one-pot purification of galactitol dehydrogenase and formate dehydrogenase, using iminodiacetic acid with chelated Co2+ modified magnetic nanoparticles as a carrier, for (S)-1,2-propanediol and L-tagatose production. Lactate dehydrogenase from recombinant Escherichia coli and formate dehydrogenase from Candida methylica are used for the regeneration of NADH/NAD+ with a representative synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol, overview. The immobilized enzyme system retained up to 70% of its activity after one week of repeated use. One-pot purification of His6-tagged GatDH and FDH followed by the production of rare sugar and chiral diol by use of affinity magnetic nanoparticles |
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