EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.B52 | A158A/N162P |
96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion |
738052 |
| 1.1.1.B52 | A158D/N162G |
96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion |
738052 |
| 1.1.1.B52 | A158D/N162Q |
95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion |
738052 |
| 1.1.1.B52 | A158H/N162G/K202Q/L224W |
mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5% |
738052 |
| 1.1.1.B52 | A158H/N162P |
98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion |
738052 |
| 1.1.1.B52 | A158K/N162M |
97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion |
738052 |
| 1.1.1.B52 | D40A |
no activity |
729146 |
| 1.1.1.B52 | D40A |
site-directed mutagenesis, inactive mutant |
741816 |
| 1.1.1.B52 | E197A |
no activity |
729146 |
| 1.1.1.B52 | E197A |
site-directed mutagenesis, inactive mutant |
741816 |
