EC Number   |
Protein Variants |
Reference |
|---|
    1.1.99.18 | C285Y |
mutation causes a 5.6time decrease in KM for glucose and a decreased affinity for the other disaccharides. Overall catalytic efficiency increases 6.7times as compared to the wild type enzyme |
762921 |
| 1.1.99.18 | C291Y |
mutation causes a 8.3times decrease in KM for glucose and a decreased affinity for the other disaccharides |
762921 |
| 1.1.99.18 | E279A |
the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity |
-, 711083 |
| 1.1.99.18 | E279D |
the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity |
-, 711083 |
| 1.1.99.18 | E279N |
the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity. The mutant retains most of its activity with cellobiose but was completely inactive with lactose |
-, 711083 |
| 1.1.99.18 | F166Y |
the redox potential of heme in the mutant is lower than that of the wild type enzyme |
673525 |
| 1.1.99.18 | H689A |
more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme |
654697 |
| 1.1.99.18 | H689E |
more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme |
654697 |
| 1.1.99.18 | H689N |
more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme |
654697 |
| 1.1.99.18 | H689Q |
more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme |
654697 |
