EC Number   |
Protein Variants |
Reference |
|---|
   1.1.99.28 | DELTA1-22/S64D |
S64D mutation converts the strict NADP+ spoecificity of wild-type GFOR to a dual NADP+/NAD+ specificity |
656483 |
| 1.1.99.28 | DELTA32-46 |
the mutant enzyme DELTA32-46 is a protein that is no longer processed but shows full enzymatic activity and has the cofactor firmly bound. The mutant enzyme DELTA2-20 or a mutant enzyme with an exchange of the entire signal sequence with the signal sequence of gluconolactonase of Zymomonas mobilis leads to an active and processed protein |
10907 |
| 1.1.99.28 | K121A |
mutant enzyme is not processed within 60 min |
655405 |
| 1.1.99.28 | K123A |
mutant enzyme shows processing behavior comparable to wild-type enzyme |
655405 |
| 1.1.99.28 | more |
mutant enzymes with deletions in the signal peptide are enzymatically active and contain tightly bound NADP(H). Mutant enzymes with a complete deletion of the signal peptide are produced as cytosolic enzymes |
10910 |
| 1.1.99.28 | S116D |
a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type |
10905 |
| 1.1.99.28 | S116D |
dual NADP+/NAD+ specificity with loss of tight cofactor binding, wild type: strict NADP+ specificity |
697992 |
| 1.1.99.28 | S116D |
significantly retarded processing kinetics with residual unprocessed form being detectable even after 60 min |
655405 |
| 1.1.99.28 | S116D/K121A/K123Q/I124K |
significantly retarded processing kinetics with residual unprocessed form being detectable even after 60 min |
655405 |
| 1.1.99.28 | Y269F |
essential acid-base catalyst, involved in substrate binding, activity completely abolished |
697992 |
