EC Number   |
Protein Variants |
Reference |
|---|
   1.11.1.23 | E142A |
complete loss of enzymatic activity in vivo and in vitro, 2.1% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105 |
701900 |
| 1.11.1.23 | F182A |
the variant favors trans-fosfomycin production from (S)-2-hydroxypropylphosphonate |
764973 |
| 1.11.1.23 | H138A |
inactive in vivo, but in vitro it retains 27% of the active site iron and nearly 20% of the wild-type activity, limited self-hydroxylation (at Tyr105) |
701900 |
| 1.11.1.23 | H180A |
complete loss of enzymatic activity in vivo and in vitro, 5.8% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105 |
701900 |
| 1.11.1.23 | Y102F |
mutant maintains 60% of the activity of the wild-type enzyme |
704157 |
| 1.11.1.23 | Y103F |
mutant maintains 60% of the activity of the wild-type enzyme |
704157 |
| 1.11.1.23 | Y105F |
mutant enzyme is inactive |
704157 |
