EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.24 | E76A |
site-directed mutagenesis of Ni-QueD, exhibits marginal quercetinase activity of 0.014 unit/mg, corresponding to a more than 10000fold decrease in specific activity, Glu76 of QueD is part of the strictly conserved cupin motif and thus is assumed to be a ligand to the metal center |
-, 702220 |
| 1.13.11.24 | E76D |
site-directed mutagenesis, the mutant retains marginal activity |
742192 |
| 1.13.11.24 | E76H |
site-directed mutagenesis, the mutation results in Ni- and Co-QueD variants that retain the native fold and show residual catalytic activity |
742192 |
| 1.13.11.24 | H69A |
site-directed mutagenesis, the mutant retains marginal activity |
742192 |
| 1.13.11.24 | H71A |
site-directed mutagenesis, the mutant retains marginal activity |
742192 |
| 1.13.11.24 | more |
generation of VdQase knock-out mutants through Agrobacterium tumefasciens-mediated transformation, phenotypes, detailed overview |
-, 742592 |
| 1.13.11.24 | more |
the metal-ligating amino acids for the structural integrity and function of Ni-QueD, the individual residues of the 3His/1Glu motif are replaced by site-directed mutagenesis |
742192 |
