EC Number |
Protein Variants |
Reference |
---|
1.13.11.58 | A451G |
the mutant reacts well with arachidonic acid even in the absence of fatty acid hydroperoxides |
729971 |
1.13.11.58 | A562G |
the product of the wild-type enzyme is 98.8% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 1.2% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (99.4%). The mutant A562G forms (11S)-hydroperoxyanandamide and 15R-hydroperoxyanandamide in the ratio 3:2 |
705464 |
1.13.11.58 | A564G |
the product of the wild-type enzyme is 99.1% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 0.9% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (71%), plus 16% (5S)-hydroperoxyanandamide. The mutant enzyme A564G forms two additional prominent products, 15-hydroperoxyanandamide (34%) and 9-hydroperoxyanandamide (19%) |
705464 |
1.13.11.58 | G567A |
site-directed mutagenesis, the mutant converts the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation |
742627 |
1.13.11.58 | I395F |
the regioselectivity of the variant is changed from the n-12 position on C20 PUFA to the n-6 position to form 15S-HFAs |
764233 |
1.13.11.58 | more |
antisense expression of Osr9-lox1 (asr9lox1), by Agrobacterium tumefaciens-mediated transformation, decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, lasmonic acid, salicylic acid and trypsin protease inhibitors |
743426 |
1.13.11.58 | more |
enzyme knockout in Oryza sativa by hpRNAi expression, transfection via Agrobacterium tumefaciens strain EHA105, in transgenic lines, r9-LOX1 expression is dramatically downregulated, compared to the nontransgenic controls. r9-LOX1 RNAi transgenic lines show 74.33% decrease in nonanal content (formed during oxidation of linoleic acid by lipoxygenase), but a 388.24% increase in acetic acid and 184.84% hexanal (direct products of 13-LOX) |
742051 |
1.13.11.58 | more |
expression of antisense coding sequence of a specific tuber LOX, the suppression mutant POTLX-1 exhibits a significant reduction in LOX activity in stolons and tubers |
742693 |
1.13.11.58 | more |
in transgenic lines, L-2, expression is dramatically downregulated, compared to the nontransgenic controls, enzyme knockout in Oryza sativa by hpRNAi expression, transfection via Agrobacterium tumefaciens strain EHA105 |
742051 |
1.13.11.58 | V569W |
the variant has a significantly lower secondx1eoxygenating activity compared to wild type enzyme |
764233 |