EC Number   |
Protein Variants |
Reference |
|---|
   1.13.12.22 | H49A |
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 13% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag |
729205 |
| 1.13.12.22 | N18A |
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 1.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag |
729205 |
| 1.13.12.22 | N18A |
the mutation suppresses the kcat value 120fold compared to the wild type enzyme |
742892 |
| 1.13.12.22 | N63A |
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 1.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag |
729205 |
| 1.13.12.22 | N63A |
the mutation suppresses the kcat value 50fold compared to the wild type enzyme |
742892 |
| 1.13.12.22 | R90E |
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 17% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag |
729205 |
| 1.13.12.22 | W67F |
kcat/Km for [4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate is 0.5% compared to the activity of recombinant wild-type enzyme with N-terminal polyhistidine tag |
729205 |
