EC Number   |
Protein Variants |
Reference |
|---|
   1.13.12.24 | A123D |
mutant shows Ca2+-dependent chemiluminescence |
744205 |
| 1.13.12.24 | C145S |
61% of wild-type activity |
746328 |
| 1.13.12.24 | C145S/C152S |
1% of wild-type activity |
746328 |
| 1.13.12.24 | C145S/C152S/C180S |
21% of wild-type activity. Contrary to wild-type, 49% residual activity in absence of 2-mercaptoethanol. The regeneration of the triple mutant aequorin is sharply inhibited by 2-mercaptoethanol |
746328 |
| 1.13.12.24 | C145S/C180S |
68% of wild-type activity. Contrary to wild-type, 28% residual activity in absence of 2-mercaptoethanol |
746328 |
| 1.13.12.24 | C152S |
46% of wild-type activity |
746328 |
| 1.13.12.24 | C152S/C180S |
17% of wild-type activity. Contrary to wild-type, 14% residual activity in absence of 2-mercaptoethanol |
746328 |
| 1.13.12.24 | C180S |
13% of wild-type activity |
746328 |
| 1.13.12.24 | D119A |
mutant is an active photoprotein, Ca2+ affinity is reduced by a factor of 20 compared to the wild type |
744205 |
| 1.13.12.24 | D153G |
shift of maximum luminescence to shorter wavelengths. t1/2 is decreased with respect to the half-life time of the native protein (0.7 s). Mutation results in a significant decrease in stability at 65°C |
764863 |
