EC Number |
Protein Variants |
Reference |
---|
1.14.11.11 | D219H |
inactive mutant enzyme |
439281 |
1.14.11.11 | D219N |
inactive mutant enzyme |
439281 |
1.14.11.11 | H217Q |
inactive mutant enzyme |
439281 |
1.14.11.11 | H66Q |
mutant enzyme has 97% of the activity of the wild-type enzyme |
439281 |
1.14.11.11 | K97A |
the shows a 3.3fold improved hydroxylation activity compared with the wild-type enzyme |
742949 |
1.14.11.11 | K97E |
the shows a 2.7fold improved hydroxylation activity compared with the wild-type enzyme |
742949 |
1.14.11.11 | K97G |
the shows a 1.7fold improved hydroxylation activity compared with the wild-type enzyme |
742949 |
1.14.11.11 | more |
enhanced production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropan alkaloid contents in transgenic lines, overview |
725946 |
1.14.11.11 | more |
functional expression in Saccharomyces cerevisiae as tagged and untagged proteins. The untagged form is about 3-fold as active as the tagged protein |
688836 |
1.14.11.11 | more |
truncation of an H6H enzyme flexible N-terminal region yields an active and stable yet more compact enzyme version |
765317 |